Laboratory for Experimental Research

The Laboratory for Experimental Research of the Department of Intensive Care Medicine supports experimental and also carries out its own studies in cell biology.

Some of the techniques used by our team are as follows:

  • Electron Microscopy/Immune Electron Microscopy 
  • Light Microscopy 
  • Enzyme Linked Immunosorbent Assay (ELISA) 
  • Cell Culture  
  • SDS Polyacrylamide Gel Electrophoresis (SDS PAGE) and Western Blotting
  • Subcellular Fractionation: Isolation of Crude Mitochondria, Pure Mitochondria and Mitochondrial Associated Membranes from Tissues or Cell Cultures
  • Determination of  Mitochondrial Respiration Rates Using High Resolution Respirometry; Determination of Mitochondrial Membrane Potential
  • Determination of Mitochondrial Respiratory Chain Enzyme Activities by Spectrophotometry and Assessment of Citrate Synthase Activity
  • Determination of Cellular or Tissue ATP contents
  • Isolation of Human White Blood Cells (T cells, B cells, monocytes) Using Magnetic beads